Can the substantiality of phenomena ever be conclusively ruled out through Buddhist practice?

I can see how one can develop, through one's practice, a very high degree of confidence in the truth of emptiness through inferring from the conditioned nature of phenomena, their impermanence, their dependence on a perceiving consciousness, and on the need for memory and intelligence in order to recognize them as existent, but is it possible to ever be 100% certain? Who's to say the Higgs Boson isn't the fundamental, truly existent building block of matter that can never be further reduced, even if quantum physics tells us it's in superposition when not interacting with an observer?